Hello Stephen, I also sort embryonic stem cells and the survival rate is around 94%, I use a 100micron and sometimes a 70 micron nozzle , for the 100 micron nozzle I use a pressure of 20PSI,the sample pressure can vary from 2 to 8 , this does not seem to make too much of a difference , we also sort straight into 6 well plates , I guess if I were in your position I would check pre and post sort viability as a first step . Ian Ian Dimmick Flow Cytometry Core Facility Manager Newcastle University Institute of Human Genetics Bioscience Centre Central Parkway Newcastle Upon Tyne NE1 3BZ UK Ian.Dimmick@ncl.ac.uk Tel 0044 191 2418831 Fax 0044 191 2418666 (mob) 0044 7970344823 ________________________________ From: Stephen Sullivan [mailto:sullivan@mcb.harvard.edu] Sent: 21 September 2006 20:52 To: Cytometry Mailing List Subject: sorting human embryonic stem cells Dear fellow FACS users, We sort human embryonic stem cells here, using a 100micron nozzle and a low rate of 1 on the Aria. Our survival rates of sorted cells are poor (2% of all sorted cells survive) and I just wondered if anyone has any suggestions or hints as to how we can improve these survival rates. Thank you very much Stephen Stephen Sullivan M.Sc., Ph.D., F.R.C.M. Harvard Stem Cell Institute SF437 7 Divinity Avenue Cambridge MA 02138Received on Mon Sep 25 12:18:00 2006
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