Florent, We used the sodium azide order from Sigma (Cat. S8032-25G). They are fine to be dissolved in PBS. Usually only 0.1% (500 mg of sodium azide in 500 mL of PBS) of sodium azide is need for a buffer to store antibodies. The buffer often include BSA. Hope that helps. Jian ********************************************** Jian Ling, Ph.D. Principal Engineer Bioengineering Section Medical System Organization Automation and Data Systems Division Southwest Research Institute Tel. (210) 522-3953 Fax. (210) 684-6147 ********************************************** ________________________________ From: florent crepineau [mailto:florentcrepineau@yahoo.fr] Sent: Monday, September 18, 2006 5:41 AM To: cyto-inbox Subject: sodium azide: how to prepare a buffer from the powder? Dear all, this is not strictly speaking a flow cytometry question but more of a chemistry question. I have purified antibodies on a column and I would like to store them in a buffer containing sodium azide. I ordered the powder but reading the MSDS, it seems impossible to use it as it is incompatible with water and explodes. How do you do to put sodium azide powder in a buffer solution? Thank you in advance. Best regards Florent ________________________________ Yahoo! Mail réinvente le mail ! Découvrez le nouveau Yahoo! Mail <http://fr.rd.yahoo.com/evt=40577/*http:/fr.promotions.yahoo.com/mail/nouveaumail.h%0d%0atml%20%0d%0a> et son interface révolutionnaire.Received on Tue Sep 19 14:58:00 2006
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