Dear Fred, I try google "SpectrumOrange" and got some piece of information for you. The peak excitation should be at around 550nm and peak emmission at around 590nm <http://www.vysis.com/images/Content/SpetrumWaveLengths_Orange.gif> >From invitrogen website, they seem have some DNA labeling kids using some dys similar to SpectrumOrange such as Alexa Fluor 546 and Alexa Fluor 555 so the excitation of SpectrumOrange is for sure around 550nm. <http://probes.invitrogen.com/lit/catalog/2/sections/9475.html> If there is no further response, you might wanna try ask the vysis (or invitrogen?) for more details. Tiger > I have a potential client who would like to use SpectrumOrange as a > label in an experiment they propose to do in our laboratory. I have no > experience with this probe. A web search, including a search of this > website, has revealed precious little information about it other than it > is often used to label chromosomes. My impression is that it is best > suited for use in fluorescent microscopy. Has anyone out this used it in > flow cytometry applications? Can it be excited using a 488nm laser? > > Any and all information about SpectrumOrange would be very helpful. > > > > Thanks, > > Fred Menendez > > Manager and Senior Research Technologist > > Flow Cytometry Core Laboratory > > Johns Hopkins Bloomberg School of Public Health > > -- Wei-Lin Tiger Bee Purdue University Cytometry Laboratories BIND122, 1203 W. State Street, Bindley Bioscience Center West Lafayette, Indiana, 47907 Tel.: 765 - 494 - 0757 E-mail: tigerbee@flowcyt.cyto.purdue.eduReceived on Mon Sep 18 12:58:00 2006
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