Hello John, I have been using the Q dots and am very impressed with them, particularly in the high extinction coefficients and the ability now to utilise my UV laser for phenotyping. The information that Invitrogen give in there explanatory booklet on Q dots situated on the website is very good and gives a lot of what you need to know , however pay particular attention to your filter set up , remember if you use the 655 Q dot then this will be excited by all lasers and will emit in all filters at or around 655 , my rationale is to use the Qdot emission that has one filter set specific to it (i.e. 610) this eliminates compensation problems. In summary a very nice flexible product, wash you samples well after Q dot incubation and I think they are a bonus to multicolour flow cytometry Ian Dimmick Flow Cytometry Core Facility Manager Institute of Human Genetics Bioscience Centre Central Parkway Newcastle Upon Tyne NE1 3BZ UK Ian.Dimmick@ncl.ac.uk Tel 0044 191 2418831/ 8825 Fax 0044 1912418666 (mob) 0044 7970344823 ________________________________ From: jtigges@caregroup.harvard.edu [mailto:jtigges@caregroup.harvard.edu] Sent: Wed 13-Sep-06 15:28 To: cyto-inbox Subject: [ Qdots setup question ] I would like to ask for help, on behalf of a researcher, on the use of Qdots on the LSR II. She is concerned with the setup and filter configurations. Any help is greatly appreciated and can be sent to myself or directly to the researcher Nilufer_Shroff@dfci.harvard.edu. Thans in advance, John Tigges Core Facility Manager Flow Cytometry Beth Israel Deaconess Medical Center 330 Brookline Avenue Boston, MA 02215 (617) 667-4901 (617) 975-5536Received on Thu Sep 14 12:58:00 2006
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