I totally agree: The Institutional Biosafety Committee has to look at this. BTW: Who has decided that the biosafety level should "only" be BSL-2 when you are handling "bugs" that are basically indestructible? My gut feeling tells me that right now nobody really has any clue about any potential longterm effects of aerosols from these specimens that you are about to generate ... Joern E. Schmitz, MD Assistant Professor of Medicine Harvard Medical School Division of Viral Pathogenesis Department of Medicine Beth Israel Deaconess Medical Center Research East 213D 41 Avenue Louis Pasteur Boston, MA 02115 Phone: 617-667-5206 Fax: 617-667-8210 http://bidmc.harvard.edu/display.asp?leaf_id=4102 http://www.hms.harvard.edu/aids/programs/cfar/cores/immflowlma.htm This document may contain information that is privileged, CONFIDENTIAL and exempt from disclosure under applicable law. If you are not the intended recipient, please notify me immediately, as the use of this information is strictly prohibited. -----Original Message----- From: Charles A Kuszynski [mailto:ckuszyns@UNMC.EDU] Sent: Thursday, September 07, 2006 11:22 AM To: cyto-inbox Subject: Re: A big thank you on biosafety I would be more concerned about the potential for aerosol production and biosafety issues than whether you can clean the sample tube. Your institutional Biosafety Committee needs to look at this project and suggest the requirements for operator safety and containment. Charles A. Kuszynski, Ph.D. Assistant Professor Director, Cell Analysis Facility University of Nebraska Medical Center 985816 Nebraska Medical Center Omaha, NE 68198-5816 402 559-6299 office 402 559-6267 lab 402 980-7654 cell 402 559-4069 fax ckuszyns@unmc.edu ***The University of Nebraska Medical Center E-mail Confidentiality Disclaimer*** The information in this email is privileged and confidential, intended only for the use of the addresse(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this email by mistake, please delete it and immediately contact the sender. Anne C Avery <Anne.Avery@ColoS tate.EDU> To Cytometry Mailing List 09/05/2006 03:15 <cytometry@flowcyt.cyto.purdue.edu> PM cc Subject Re: A big thank you on biosafety Hello, An investigator here has inquired about using the MoFlo for sorting prion infected material. This will be from deer and elk with chronic wasting disease, which is treated as BSL2, and may eventually also be scrapie, which I think is also BSL2. I was wondering if anyone out there has done these kinds of sorts, and if so, what sort of containment and line decontamination they have used? Thanks very much. Anne -- Anne Avery, VMD, PHD College of Veterinary Medicine and Biomedical Sciences Department of Microbiology, Immunology and Pathology 1619 Campus Delivery Fort Collins, Colorado 80523-1619 voice:(970)491-1170 fax:(970)491-0603 anne.avery@colostate.eduReceived on Mon Sep 11 16:18:00 2006
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