Date: Thu Sep 07 2006 - 04:06:45 EDT
Hi Brian, I would guess that unless reagent choises have become better recently, you're in for a world of pain. From working on cytokine production assays ages ago I remember most/all anti-TGFbeta's are highly species cross-reactive, so any trace of serum (say FCS) in FACS stain buffer and/or cell culture medium just prior to FACS can cause spurious positive signals. Yes, that's unlikely to be intracellular but the bottom line is: in the presence of high background signals, even data from the best controlled experiments can be treated as suspect afterwards. Is there a fundamental reason in your experiment not to take advantage of the TGFbeta associated protein LAP (latency associated peptide, if I recall correctly)? I saw a bunch of presentations by Howard Weiner (Boston - say a publication recently as well) a while ago, and he's detecting TGFb producing cells by using an anti-LAP mAb. LAP gets cleaved off TGFb before TGFb becomes active, so that's something to keep in mind when interpreting the data - you're detecting the inactive form of TGFb. Best of luck, Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Brian Newsom [mailto:bnewsom@opexatherapeutics.com] Sent: Tuesday, September 05, 2006 9:36 PM To: cyto-inbox Subject: TGFbeta Does anyone know of a source for an anti-TGFbeta antibody for ICC? Thanks, Brian Brian Newsom Senior Director of Project Development Opexa Therapeutics (OPXA.OB) 2653 N. Crescent Ridge The Woodlands, TX 77381 (281) 775-0627 - Direct (281) 775-0609 - Main (281) 775-0662 - Private Fax <mailto:bnewsom@opexatherapeutics.com> bnewsom@opexatherapeutics.com <http://www.opexatherapeutics.com/> www.opexatherapeutics.com "The capacity of one's mind is directly proportional to the amount of coffee they consume." ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
