Hello Flowers, As usual when a difficult question comes up, this forum is the best place to get answers. I'm getting conflicting results when trying to replicate flow cytometry based experiments for antibody-dependent cell mediated cytotoxicity using CFSE as the "viability dye" and PKH as the tracking dye. On the other hand, the Guava CellToxicity® Kit uses CFSE as the tracking dye and 7-AAD as the viability dye. This is the first source of confusion for me. Since CFSE covalently binds to intracellular proteins upon uptake, I tend to favor the use of CFSE as a tracking dye. Looking at the literature, I found a paper that investigated the fate of CFSE after apoptosis/necrosis and they state that CFSE is lost from the cytosol but can be maintained in the nucleus. So I'm confused further (or maybe that's the definitive answer!). Is this phenomenon cell/cell-line dependent? Would anyone care to chip in with their 2¢? Thanks all! Maloy Mangada, PhD Scientist, Cell Biology Acambis, Inc. 38 Sidney St., Cambridge, MA 02139 -------------------------------------------------------- The contents of this e-mail are confidential and may be privileged. This e-mail is intended for the addressee(s) only. Any unauthorised use may be unlawful. If you receive this e-mail by mistake, please advise the sender immediately. Please visit our website at www.acambis.comReceived on Fri Sep 1 12:38:00 2006
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