Re: [ small fluorescent molecule question ]

Hi Matt,

The Alexa dyes (Molecular Probes/Invitrogen) are small molecules easily
purified away from the bound form.  Cy dyes and Texas Red also fall into
this category, but the Texas Red is normally not used by itself as it needs
a laser at a wavelength not available on most instruments.  The Alexa dyes
come in convenient easy to use kits or as bulk (which is what I get and
saves a bit of money).

Good luck,

Randy T. Fischer, NIH/NIAMS
B Cell Biology Group
9000 Rockville Pike
Bldg 10, Room 6D50
Bethesda, MD 20892
(301) 594-3537 (voice)
(301) 402-2209 (fax)


> From: "Matthew J. Ford" <mjford@brc.ubc.ca>
> Date: Mon, 28 Aug 2006 14:43:46 -0700
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> 
> Hi everyone,
> 
> Does anyone have a list of small fluorescent molecules that have be
> conjugated to antibodies?  I am interested in those that can be
> purified away in a simple manner, (eg dialysis).  FITC falls into
> this category...biotin too (although not fluorescent)...any others
> that have been done?	It is not trivial to purify away excess PE/APC
> etc.
> 
> Thanks in advance,
> 
> 
> Matt Ford
> Antibody Facility Manager
> Biomedical Research Centre
> University of British Columbia