Re: The folklor based HTS

Hello Akos,
click on the experiment in browser, then go to inspector (experiment 
tab) and disable the "Use global instrument settings" option. Than it 
starts to work. You can paste different instrument settings on each well 
of the plate (protocol tab). If you want to edit the instrument settings 
for particular well, the well has to be activated on collection tab. Our 
Diva version is 4.1.2. If I remember correctly, the "Use global 
instrument settings" option was introduced to DIVA in versions 4.1 +.
Btw, Diva 5 is already out? I have inquired our local BD representatives 
some time ago and didn`t got the answer yet. Is it a free or paid upgrade?
Best regards
Ondrej

-- 
********************************
Ondrej Horvath M.D.
Laboratory of Molecular Immunology
Institute of Molecular Genetics
Videnska 1083
14220 Prague
Czech Republic
--------------------------------
Tel. +420-241062589
Fax. +420-244472282
e-mail: horvath@biomed.cas.cz
********************************



akos.szilvasi@novartis.com wrote:
>
> I am a great fan of the HTS on either the Calibur or the LSR II. I 
> have	- I think - purchased 5 or 6 of them in the last few years. They 
> are elegantly designed, reliable and productive attachments to the 
> cytometers.
>
> However, when it comes to its operation we are on our own. I have yet 
> to see a user guide for the Diva version of the HTS (the Diva 5 manual 
> conveniently ignores the HTS part of the software) or at least find a 
> wise man who can tell me how to do certain tricks. Unlike on the 
> Macintosh platform, the Diva version - though much more user friendly, 
> clean and simple - lacks flexibility. In the menus there are 
> instrument setting copy and paste, export and import functions but 
> they do not work. I may be doing something wrong, but what?
>
> Is anyone able to preprogram different wells for different 
> experiments? In real life we often have multiple cell lines on the 
> same 96 well plate. Currently the only way to run them is to acquire 
> one cell line, stop the experiment, change the settings, start the 
> next cell line, stop the experiment...etc. This is in both, Diva 4 and 
> Diva 5.
>
> I figured out that you can load "setup" parameters and assign them to 
> individual wells. This feature is rather a programming mistake than a 
> feature because you can not copy and past or other way assign that 
> settings to other wells in the group. The "setup catalog" is a 
> collection of compensation and other setting parameters that you only 
> can generate when saving a comp setting. Not a practical way of 
> creating the setting file.
>
> If anyone is more advanced in HTS usage, please, share your experience 
> with me or send me a pdf user guide for Diva 5.
>
> Thanks, Akos
>
> ________________________________________________
>
> Akos Szilvasi
>
> Flow Cytometry and Confocal Microscopy Core Facilities
> Novartis Institutes for Biomedical Research, Inc. (NIBRI)
> 100 Technology Square, Cambridge, MA 02139
> akos.szilvasi@novartis.com
> tel 617 871 7177
> fax 617 871 7051
>
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