Hi Flowers, I have a question about the FoxP3 antibody. I have tried using eBioscience human foxp3 abs without success. We got one of their APC Anti-Human FoxP3 kits and followed the exact protocol given by them and it still would not work. I tried the experiments with both whole blood and PBMCs with similar results. In fact, the expression was even below the Isotype control. Any suggestions/experience with foxp3. I will greatly appreciate it if anyone can send me a protocol with which they had success. Thanks , Vijay
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