Similar behavior occasionally happens on our Calibur, although it is not usually limited to one parameter. Each time this has been traceable to a dirty flow system, especially the sample uptake tube. A thorough cleaning with bleach fixes this for us. Because of the different paths in the emission optics, a slight change in sample path due to crud in the sample tube, or on the tip of the tube, could affect one parameter more than others. Marty Marty Bigos Director, Flow Cytometry Core Laboratory Gladstone Institute of Virology and Immunology Mail & Deliveries: 1650 Owens Street Rm 521 San Francisco CA 94158 (tel) 415-734-4821 (fax) 415-355-0855 (mobile) 415-845-8450 mbigos@gladstone.ucsf.edu http://www.gladstone.ucsf.edu http://www.gladstone.ucsf.edu/gladstone/php/?sitename=flowcytometry At 4:52 PM +0100 8/10/06, Michael Hughes wrote: >Hello All, > Can anyone suggest an explanation for the following problem >I saw on the Calibur this morning. >One of my users had lymphocyte samples stained with 4 coloursto run >on the Calibur. The Fl1 and Fl2 signals were fine but the FL3 signal >drifted up over the course of about 1 minute and then became stable. >The Fl3 signal (PECY5) was originally below the baseline and came up >to the correct position in the 1st Quadrant. I checked some beads on >the Calibur and all 4 signals were fine and showed no drift. The >same sample run on the Vantage did not drift but came to the correct >levels on all PMT's almost instantaneously. We haven't seen this on >similar samples before. > >Thanks for any help >Mike Hughes, Flow Cytometry Manager, PICR, Manchester > > --Received on Mon Aug 21 12:58:02 2006
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