JM, Go to the Perdue website and do a search for fixation and PFA. You will find many good discussions. Two great reviews that address fixation by Jacobberger & Bauer listed below if you want to get into the details. ---- Jacobberger JW. Stoichiometry of immunocytochemical staining reactions. Methods Cell Biol. 2001;63:271-98. Bauer KD, Jacobberger JW. Analysis of intracellular proteins. Methods Cell Biol. 1994;41:351-76. Calman From: Garcia JM <jmgarcia@hkucc.hku.hk> Date: Thu, 27 Jul 2006 10:31:09 +0800 To: cyto-inbox Subject: [ Fixation Methods ? ] Dear all, I am new in that mailing list. Appart of PFA and cold methanol fixation. Is there any other fixation methods? Furthermore, when looking at litterature, i have the feeling that the method used is more a question of habit that with real rational (why one or another, why 2% versus 3% PFA, etc). of course i know it is caricatural because, depend on what one is looking on the cell a method would be better, and there is some aspect like PFA that may disturb reading on DsRed). I know my question is very naive but i would appreciate you advice on that matter. Thanks a lot in advance. Best regards JM GarciaReceived on Mon Aug 14 12:38:00 2006
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