Hey Janet, DRAQ5 will penetrate live cells. It is best excited at 633nm, but seemingly, can also be excited at 488nm. Produced by Biostatus http://www.biostatus.co.uk/draq5_technical.html If you can excite by a UV laser, Hoechst 33342 is an alternative. Regards. Vladislav Rozenkov -----Original Message----- From: janet dow <jldow@unity.ncsu.edu> To: cyto-inbox Sent: Fri, 4 Aug 2006 14:49:05 -0400 Subject: sorting cancer cells for ploidy Hey Fellow Flowers: I have a client who wants to sort canine cancer cells based on ploidy and then grow them in culture. I don't have any experience with this. She is specifically looking for a protocol that will keep the cells alive and happy yet stain the DNA. Thanks in advance for all your help. Janet -- Janet Dow Laboratory Research Specialist and Manager Flow Cytometry and Cell Sorting Facility North Carolina State College of Veterinary Medicine 4700 Hillsborough Street Room C-309 Raleigh, NC 27606 (919)513-6443Received on Tue Aug 8 11:58:00 2006
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