Re: FlowJo and DiVa file IDs

Gayle,

I've learned that if you export as 'Experiment', you get all of the data 
including settings, but you have numbers instead of sample IDs as you 
designated them during acquisition, e.g., 'T4', etc.

If, on the other hand, you export as 'FCS files', you can see the sample 
IDs you designated, but (at least they say - I haven't looked) you lose 
some of the info about your sample such as some settings.

If you are using only your DiVa computer for analysis of archived files, 
you should be OK with 'Experiment'-exported, since these files, when 
retrieved in DiVa on that computer, will still have the sample IDs as 
well as all the other info.

If you are analyzing archived files using FlowJo (as we are trying to 
do), however, then files exported as 'Experiment' will have those, 
pardon me, damnable numbers instead of the IDs they were saved as 
originally.

I 'm going to try and buy another copy of DiVa software/dongle from BD 
and install it on the offline computer on which we are running FlowJo.	
Then I'm going to export everyone's files to a CD as 'Experiments' from 
the 'primary' DiVa computer.  They will then bring the files up for 
analysis either using DiVa on the second computer, which won't require 
any extra manipulation, or they can bring them up in DiVa and re-export 
as FCS files for FlowJo (so nice to be able to overlay histograms using 
click-and-drag, the way FlowJo makes it possible!).  Not exactly easy 
but hopefully it will work.

There is another way to find the sample IDs of 'Exeriment'-exported 
files when using FlowJo (in FlowJo under preferences, I believe) but I 
found it to not be easy.

Hope this helps.

Ray Hester
The Univ. of South Alabama

Gayle Thornbury wrote:

>I tried this, but the FCS file names show up as 4 digit numbers, not the names that I
had
>given them.  Is there something that I'm missing?
>Gayle Thornbury
>Terry Fox Laboratory
>Vancouver, BC
>
>________________________________
>
>From: Ian Dimmick [mailto:Ian.Dimmick@newcastle.ac.uk]
>Sent: Wed 7/19/2006 12:42 AM
>To: cyto-inbox
>Subject: RE: FloJo and DiVa file IDs
>
>
>
>This is very easy, all you do is when the experiment selected go to the
>file menu at the top of the screen and in the drop down menu there is an
>option to export experiment, you then select the location and you then
>have a copy of your experiment filled with your FCS files , the
>compensation matrix and instrument settings will form part of your
>experiment as well as your FCS files
>
>Ian
>
>
>Ian Dimmick
>Flow Cytometry Core Facility Manager
>Institute of Human Genetics
>Bioscience Centre
>Central Parkway
>Newcastle Upon Tyne
>NE1 3BZ
>UK
>
>Ian.Dimmick@ncl.ac.uk
>Tel	 0044 191 2418831
>Fax	0044 191 2418666
>(mob)	0044 7970344823
>
>-----Original Message-----
>From: Ray Hester [mailto:rhester@jaguar1.usouthal.edu]
>Sent: 17 July 2006 21:17
>To: cyto-inbox
>Subject: FloJo and DiVa file IDs
>
>Can someone tell us how to export DiVa files as 'Experiments',
>not as 'FCS files', so that the individual tubes within the
>'Experiment' retain their specific identities, e.g., 'T4/8',
>rather than some numerical value, e.g., '2437'.  When we export
>DiVa data as 'FCS files', the specific tube ID is retained.
>
>We have a FloJo 7.1 USB dongle and it's being used primarily on PCs.
>
>Thanks.
>
>Ray Hester
>Univ. of South Alabama
>
>
>
>
>  
>