We've traditionally made up 7-AAD in 95% ethanol and stored it at -20C - ethanol is still liquid at this temperature, so you do not need to thaw it to use it (like you would for DMSO). I expect methanol would behave the same. In our hands, 7-AAD seems to work for quite a while as a viability stain when stored this way, but its ability to stoichiometrically bind DNA seems to decline after extended storage (1 year). Enjoy, Bill Telford NCI-NIH ________________________________ From: arjmand [mailto:arjmand@farabi.tums.ac.ir] Sent: Sunday, July 23, 2006 11:45 PM To: cyto-inbox Subject: 7-AAd storage Dear Friends, Solutions of 7-AAD is said to be made in DMSO and/or absolute Methanol. Basically why this DNA prob (I prepared in Methanol) shouldn't be fluorescent after a year? and is there any other solvent for longer storage? Regards, Alireza Ardjmand Hematology, Oncology and B.M.T research Cent. Shariati Hospital Kargar Ave. 14114 Tehran-Iran www.tums.ac.ir <http://www.tums.ac.ir/> -- This message has been scanned for viruses and dangerous content by MailScanner <http://www.mailscanner.info/> , and is believed to be clean.Received on Tue Jul 25 12:38:00 2006
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