Alireza, We routinely use 7AAD for viability determination on thawed PBMC. We resuspend the lyophilized powder (1mg) with 50ml sterile DPBS (it is difficult to get into solution, but it will go) aliquot and store at 20ēC for years. We stain cells with approximately 5ug/ml for 15min. Once an aliquot is thawed, we store it in the fridge for up to 2 weeks and see little change in fluorescent intensity. Not sure if this preparation will work for other applications, but for us, it works great! Good luck! Shannon McGrath, MS Non-Human Primate Laboratory Malaria Vaccine Program Walter Reed Army Institute of Research 301-319-9727 On 7/23/06 11:44 PM, "arjmand" <arjmand@farabi.tums.ac.ir> wrote: > Dear Friends, > > Solutions of 7-AAD is said to be made in DMSO and/or absolute Methanol. > Basically why this DNA prob (I prepared in Methanol) shouldn't be fluorescent > after a year? and is there any other solvent for longer storage? > > Regards, > > > Alireza Ardjmand > > Hematology, Oncology > and B.M.T research Cent. > Shariati Hospital > Kargar Ave. 14114 > Tehran-Iran > > www.tums.ac.ir <http://www.tums.ac.ir/>Received on Tue Jul 25 12:18:00 2006
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