Hi Caroline, Clontech has a large selection of fluorescent proteins, and I recall having seen tables describing optimal colour combo's. Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Vergette, Caroline [mailto:cvergette@ohri.ca] Sent: Thursday, July 20, 2006 3:30 PM To: cyto-inbox Subject: Multiple fluo proteins on MoFlo Hello, A researcher at our facility is interested in detecting two, maybe three different fluorescent proteins simultaneously. I am having difficulty suggesting possible combinations from the wide array of fluorescent proteins presently available. Our MoFlo is equipped with 355, 488 and 635 nm excitation lines. Does anyone have experience with this kind of experiment? Thanks for your help Caroline Vergette Flow Cytometry Facility / Service de cytométrie en flux Ontario Genomics Innovation Centre Ottawa Health Research Institute / Institut de recherche en santé d'Ottawa 501 Smyth Road Ottawa ON Canada K1H 8L6 Tel: 613-737-8899 ext. 73916 Fax: 613-737-8803 www.ottawagenomecentre.ca <http://www.ottawagenomecentre.ca/> ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
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