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From: "Eric Dudl" <epdudl@hotmail.com>
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Subject: Calcineurin levels / activity by flow cytometry?
Date: Thu, 20 Jul 2006 16:31:11 +0000
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Hi.

I was wondering if anyone has had experience measuring calcineurin levels or 
activation status using flow?  My goal is to measure whether certain 
compounds can reduce total calcineurin or change it's phosphorylation 
status.  So far, all I've seen for it involves western blotting.  Does 
anyone here know if it can be done with cytometry?

Thanks in advance,


Eric Preston Dudl
Postdoctoral scientist
John Reed Laboratory
Program in apoptosis and cell death research
The Burnham Institute for Medical Research
10901 N. Torrey Pines Rd, Building 6, Rm 6308
La Jolla, CA 92037
858-646-3100 x. 4322
edudl@burnham.org