Date: Thu Jul 20 2006 - 04:20:43 EDT
Hi Mark, I can sympathize - but let's be creative: I've used irrelevant target specific humanized mAbs (say, cancer specific one on freshly isolated leucocytes) as negative controls for isotype matching humanized ones that binds surface markers (surface receptors, CD's, ...). You could also think about using a soluble protein specific one as a negative control for a surface specific one (say, anti-TNF, but be careful with that one - there's documentation for surface expression for some of this type of targets). You can get most of these from your local pharmacist, as there are plenty of humanized monoclonals in the clinic already - just try to find a doctor to write you a script where needed, or beg for leftovers at your local hospital pharmacist (many are administered in the hospital as infusions. Once reconsituted/thawed, most of these drugs cannot be used later on, as per product insert administration procedures. Since most to all are dosed according to the patient's body mass, not as a fixed amount, there's bound to be some stuff left over in the vial - which is good enough for science if kept at 4C, but certainly not for use as an i.v. drug). Alternatively, and very accessible/cheap to boot, you could just purchase some human plasma isolated polyclonal IgG1 from Sigma! have fun, Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Mark D'Amico [mailto:MDAmico@panaceapharma.com] Sent: Wednesday, July 19, 2006 2:59 PM To: cyto-inbox Subject: Control Abs Hello, again, As I said in the earlier post, I have a humanized antibody for use in flow. Can I use an antibody(ies) raised in mice, or unicorns for that matter, as isotype controls????? I am having trouble (as you might expect) finding human abs. He'p me, please. Mark D'Amico, Ph.D. Scientist Panacea Pharmaceuticals 207 Perry Parkway, Suite 2 Gaithersburg, MD 20877 Phone: 240.243.8000 x206 email: mdamico@panaceapharma.com A good deal of tyranny goes by the name of protection.~Crystal Eastman ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
