Where are the ( aggregated) nano-particles?

We know from our microscopic observations that our nan-oparticles ( ~ 70 nm
or aggregates, non-fluorescent) are avidly taken up by our cultured cells.
Looking by flow and thresholding on the nuclei ( LDS-751 stained, FL3
shiny), we see a great change in side scatter  when cells have been cultured
for about a day in the presence of, and taken up, the nano-particles . The
nano-particles ( & their aggregates) alone are not visible when tresholding
on FL3. 


However, the control experiment, where we add the particles to the cells &
look right away --- is giving us the same high side-scatter profile as when
the cells have had the time to internalize the particles.


So my question: IS this telling us that the particles bind very quickly - or
- that the increase in SS is merely a reflection of the fact that the
particles are suspended in the same, cell-containing droplet, thus
triggering as an LDS-positive, light scattering event?


Many thanks,

Snezna



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