Dear Rich: We have some satisfactory experience with 200 u nozzle (thanks to Wayne Wright(!), former BD) Stability is the major problem... So: 1/ be sure that you have a lot of sheath liquid in the tank when you are starting; 2/ start setup with slightly higher pressure (10-11 psi) and go down to 4-6 psi for the sort; 3/ unstability may be related with air in the nozzle...if you get out air and want to try again...always re-start everything from zero; 4/ keep speed low (less than 1000 events/sec); 5/ sheath liquid going to run out faster, and when level of liquid in tank goes too low, you may lose stream stability... Hope, it helps, Natasha -----Original message----- From: "Konz, Richard" Richard.Konz@umassmed.edu Date: Tue, 27 Jun 2006 11:54:45 -0400 To: cyto-inbox Subject: 200 um Nozzle Sorting > > Greetings everyone: > > Is anyone out there sorting on a Vantage SE DiVa with a 200 um nozzle? > Any tips or words of wisdom would be appreciated. > > Best regards to everyone, > > -Rich > > > > Dr Natasha Barteneva Director, CBRI Flow Cytometry Core, Harvard Medical School 200 Longwood Ave, #470 Boston 02115, MA phone: 617-278-3037 fax: 617-278-3167 e-mail: barteneva@cbr.med.harvard.eduReceived on Thu Jun 29 11:18:00 2006
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