RE: canine Abs

A quick question related to canine immunophenotyping:  
 
I was once told that flow cytometry evaluation of canine lymphoma has limited clinical utility, since most dog lymphomas are T-cell malignancies, hence the results are often inconclusive.    Does anyone have current data about the relative distribution of B vs T cell lymphoma in dogs, or about the utility of flow cytometry in routine canine lymphoma evaluation?	 And is there a reason that canine kappa and lambda antibodies have not come to market?
 
Thanks,  
Mike Suter
Oregon Medical Laboratories
 
-----Original Message-----
From: MConrad [mailto:conradml@gmail.com]
Sent: Thursday, June 22, 2006 1:29 PM
To: cyto-inbox
Subject: Fw: canine Abs
 
Dave.
 
Peter Moore from the University of California has antibodies to TCR ab (CA15.8G7), TCR gd (CA20.8H1) and CD3 (CA17.2A12) that work quite nicely.
 
His email is pfmoore@ucdavis.edu
 
Cheers
 
Melanie Conrad, PhD.
Centre for Biomedical Research - University of Victoria
Victoria BC, CANADA
(250) 472-4072
----- Original Message ----- 

From: David.C.McFarland@gsk.com 
To: cyto-inbox
Sent: Wednesday, June 21, 2006 5:53 AM
Subject: canine Abs
 

Good day all.  I'm having some trouble locating Abs for canine leukocytes.  I did find a few useful ones at VMRD, but I can't seem to locate a CD3.  Also, they have a "CD45-like" as opposed to just CD45.	I've used their "neutrophils" ab before with great success, but I have no experience with others.  I've also considered just trying anti-human abs from the usual suspects as I know that more often than not that works for our histo folks.  I would appreciate any info from those of you that have dog experience, especially insight into apparent lack of CD3 and "CD45-like" vs true CD45. 

Thanks, 

Dave 

David McFarland
Principal Scientist
GlaxoSmithKline

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