macrophage autofluorescence

Hello,


I have significant auto-fluorescence from cultured/infected/cytokine treated
monocytes/macrophages that inhibits my ability to see anything by flow.  I
have seen some methods for quenching macrophage auto-fluorescence on the web
and was hoping that if any of you have had experience with any of these
methods you wouldn't mind to send me a quick response regarding what
method(s) you find to be most effective.  Also, would the use of a quenching
dye, such as crystal violet or trypan blue affect subsequent intracellular
staining?


Thanks in advance!


Tracy Fischer-Smith, PhD

Temple University

Dept. of Neuroscience

Center for Neurovirology

Philadelphia, PA