Cell size using FSC is a very reliable marker of B cell activation. For staining follicular B cells , we use classII (increases dramatically) and CD5. good luck, Rachel ======================================================= Rachel M. Gerstein, Ph.D. Associate Professor Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) -----Original Message----- From: Agudelo, Marisela [mailto:magudelo@health.usf.edu] Sent: Tue 6/20/2006 10:54 AM To: cyto-inbox Subject: cell activation/differentiation markers Thanks for your responses. Attached is a great review of B cell development from the Scripps Research Institute (courtesy of Matt) Thanks Marisela Agudelo Graduate Research Assistant University of South Florida Question: Dear Flow users, I am currently isolating mouse splenic B cells and culturing them with different activators. I stain for CD19, IgM and IgE. My main question: are there any specific mouse B cell markers that can help me differentiate between naïve and activated B cells, or perhaps a memory or plasma cell marker? I would like to see if there are any changes of surface expression of "X marker" on B cells after activation and treatment with different drugs. Thanks, Marisela Answers: CD138 is a plasma cell marker, and CD27 a good memory marker, and CD69 is a good activation marker for B cells. Check out the attached review Matt CD23 is considered a good activation marker for murine B cells and CD138 as at least a partial marker for plasma cells. CD23 goes down on activated B cells while CD138 is present on many plasma cells (I won't say all PC are CD138+ as I have seen no evidence that this statement is true). Randy T. Fischer, NIH/NIAMSReceived on Thu Jun 22 12:53:41 2006
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