I want to determine in what stage of the cell cycle particular primary lymphocytes are dying. So ask the community if anyone has a recommended protocol for CELL CYCLE (DNA content with BrdU incorporation to properly distinguish all cell cycle phases) with TUNEL, or another well established method for assaying for apoptosis. Or can sorting of populations in the different stages of the cell cycle be performed? I am experienced with sorting, so is it a problem sorting different populations when aquiring data on a linear scale (like when using PI, 7AAD, or DAPI for cell cycle? Would the community recommend just sorting different lymphocytes based off their cell cycle, the subsequently enumerate apoptosis by microscopy, or look at the degree of DNA fragmentation on a gel. Any opinion on such a task would be greatly appreciated. best, Matt -- Matthew R. Janes, MSPH Ph.D. Candidate Department of Molecular Biology & Biochemistry Center for Immunology University of California at Irvine mjanes@uci.eduReceived on Thu Jun 22 12:13:42 2006
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