Research Triangle Cytometry Association Meeting Announcement Activation-State Analysis in Single Cells: Techniques and Clinical Applications Robert Balderas VP of R&D BD Biosciences Intracellular assays of signaling systems have been limited by the inability to correlate functional subsets of cells in complex populations on the basis of active protein states within the native context of the cell. We demonstrate the ability to simultaneously monitor active protein states via phospho-epitope recognition in subpopulations of complex cell populations by multiparameter flow-cytometric analysis. Multi-dimensional assessment of active protein states, in combination with surface marker and other flow cytometric detectable parameters (i.e., cytokines, apoptosis), can provide functional assessment on a single cell level that may have utility in clinical diagnostics and/or disease progression. Furthermore, the ability to profile both activating and inhibiting conditions of multiple protein states simultaneously within the cell in a rapid and parallel manner may be extended to pharmaceutical screening of compounds. and Practical Considerations for Polychromatic Flow Cytometry Mark Kukuruga BD Biosciences Manager Technical Applications Group Tuesday, June 20, 2006 10:30 AM - Noon Duke University Jones Building Room 143 Durham, North Carolina Directions from RTP: Take Alexander Drive to the Durham Freeway (147). Head North toward downtown Durham and take the Trent/Elba exit. Go straight and bear left into the Parking Garage II across from the Medical Center. Parking will cost $1.00/hr but no more than $6.00. Directions from Parking Garage II: Cross Erwin Rd. and enter Duke University Medical Center through the front door. Proceed straight ahead toward the elevators past the information booth on the right. Take a right at the elevators and proceed toward the Cafeteria (?The Atrium?). Pass the Atrium on your right and straight in front of you will be the Department of Medicine Office 1101. Bear to the right and proceed down the causeway. Go through the double doors and past the elevators and continue straight into the CARL Building. Go straight through the glass door out onto the breezeway and take a right. Take the first door on the left and enter the Jones Building. Take the stairwell to your right and go down 1 flight of stairs to the first floor. Take a right and go straight down the hall. You will run right into Jones 143. We gratefully acknowledge BD Biosciences for their support of this meeting. For more information about this meeting contact: John Whitesides jwhtsds@duke.edu (919) 684-4895 For information about the Research Triangle Cytometry Association contact: Marie Iannone marie.a.iannone@gsk.com (919) 483-9486 To be added or removed from the RTCA mailing list contact: Sam Witherspoon sam.m.witherspoon@gsk.com (919) 483-3078Received on Sat Jun 10 17:58:00 2006
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