Dear Flowers, I wanted to know how people stain for several antibodies at the same time when the antibodies have been previously diluted. For example, human antibodies from beckman coulter come 20ul/test, so if I stain for several antibodies at the same time (in the same well) my experience is that the antibodies get diluted too much and the % of stained cells is not the same as if I would have stained with a single antibody per well. Has anybody else noticed this problem and what can I do to solve this issue. Thank you very much for your help, Jenny ZilberbergReceived on Thu Jun 8 11:18:00 2006
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