Re: Magnetic cell sorting

Dear (MAC) sorters,

please have a look again at the first publications describing the MACS System:

Miltenyi, S., Muller, W., Weichel, W. & Radbruch, A. High gradient
magnetic cell separation with MACS. Cytometry, 231-238 (1990).

Even in those early days recoveries of 90% were obtained. In that
publication, we used spleenic the CD4 positive cell population to
demonstrate depletion and the B220 positive cells for enrichment.  In
this latter case we optained a recovery of more than 90% and a purity
of 98 %.

Yours
Werner Müller
(now at GBF, Braunschweig).


2006/5/15, Boris Fehse <fehse@uke.uni-hamburg.de>:
>
>
> Dear (MAC) sorters,
>
> I hope we won't be confronted with a series of ads and sarcastic letters
> from different companies involved in magnetic cell sorting. Obviously all
> systems have their +es and -es. (We have tried quite a number of them...)
>
> Best regards
> Boris
>
> p.s. Sorry for that, Jenny - I checked our data and in our hands it's no
> problem to sort CD4 and CD8 with high purity (>90%) with Miltenyi beads.
> (I'm not with the company, and have no any commercial interest). ...;) B.
>
>
> Boris Fehse, PhD
> University Medical Center Hamburg-Eppendorf
>
>
>
>
>
>
>
>
> -----Ursprüngliche Nachricht-----
> Von: Jenny Cabanillas [mailto:cabanillas@optonline.net]
> Gesendet: Donnerstag, 11. Mai 2006 21:43
> An: Cytometry Mailing List
> Betreff: FW: Magnetic cell sorting
>
>
>
>
> Dear Roger,
>
> I don't believe we had the pleasure of meeting.  I am part of the Invitrogen
> CSA team, and I'm responsible for the Dynal Beads in NJ/NY.
>
> It amazing that you would say 90% purity and recovery since from my
> canvassing I have heard from researchers that the results vary incredibly
> from
>
> Experiment to experiment (that's if the columns don't clog of course).  It
> varies from applications but over 90% is a bit high. I would check my data
> if I were you J
>
>
>
> Proof is in the pudding! Having fun selling Dynal beads,
>
> Jenny Cabanillas-McCauley
>
>
>
>
>
>
>
>
>
> -----Original Message-----
> From: Roger Burger [mailto:rburger@miltenyibiotec.com]
> Sent: Tuesday, May 09, 2006 5:44 PM
> To: Cytometry Mailing List
> Subject: RE: Magnetic cell sorting
>
>
>
> Hello Beverly
>
> I hope you meant to say,
>
> "Hah, you are lucky, for mouse the yield is more like 10% and that's what
> Dynal tells you to expect with their system!"
>
>  With the Miltenyi System and MACS(r)  CD4 and CD8 Microbeads you can achieve
> >90% purity and >90% recovery.
>
> Regards,
>
>
>
> Roger A. Burger, Ph.D.
> Technical Marketing Scientist
> Miltenyi Biotec, Inc.
> 12740 Earhart Ave.
> Auburn, CA 95602
> Phone: 530-887-5351
> Fax: 530-745-2851
> www.miltenyibiotec.com
>
>
>
>
>
> -----Original Message-----
> From: Beverly Barton [mailto:bartonbe@umdnj.edu]
> Sent: Tuesday, May 09, 2006 5:00 AM
> To: Cytometry Mailing List
> Subject: Re: Magnetic cell sorting
>
>
>
> Hah, you are lucky, for mouse the yield is more like 10% and that's what
> Miltenyi tells you to expect with their system!
>
>
>
> Sad but true.....
>
>
>
> Beverly
>
>
>
> Beverly E. Barton, Ph.D.
> Assistant Professor
> Department of Surgery/Division of Urology
> UMDNJ-NJMS MSB G519
> 185 S. Orange Avenue
> Newark, New Jersey 07103
>
>
>
> Telephone 973-972-0662
> E-mail bartonbe@umdnj.edu
> Telefacsimile 973-972-3892
>
>
>
>
>
>
> On May 6, 2006, at 3:50 AM, RAJA ALAMELU wrote:
>
>
>
> Dear Flow list members,
>
> My question is not directly related to flow cytometry, but now and then I
> have seen questions in other areas also pop up.
>
>
> We are attempting to do CD4 and CD8 cells positive isolation from human
> PBMC, followed by Detach-a bead step, using Dynal magnetic beads. We have
> followed the manufacturers instructions as it, is as well as varied each of
> the conditions for optimization.
>
>
> While the purity and viability after separation seem to be good, the final
> cell yield never crosses ~30% of the total available CD4/CD8 in PBMC. Also
> the initial input number, magnet bound cells and unbound cells do not tally.
>
>
> Is 25-30% the commonly expected yield using this technique? If anybody has
> observed better yield, purity and viability, can you please share the
> procedure?
>
>
> Thanking you in advance,
> Alamelu Raja
>
>
>
> Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates
> starting at 1¢/min.


-- 
--
Dr. Werner Müller
Department of Experimental Immunology
GBF mbH
Mascheroder Weg 1
D-38124 Braunschweig
http://www.gbf.de/ei/

Phone: +49-531-6181-303
Fax: +49-531-6181-444

I prefer to be contacted by Email
Fast contact to wmugbf@gmail.com
My email address is wmueller@gbf.de