AW: Magnetic cell sorting

RE: Magnetic cell sortingDear (MAC) sorters,

I hope we won't be confronted with a series of ads and sarcastic letters
from different companies involved in magnetic cell sorting. Obviously all
systems have their +es and -es. (We have tried quite a number of them...)

Best regards
Boris

p.s. Sorry for that, Jenny - I checked our data and in our hands it's no
problem to sort CD4 and CD8 with high purity (>90%) with Miltenyi beads.
(I'm not with the company, and have no any commercial interest). ...;) B.


Boris Fehse, PhD
University Medical Center Hamburg-Eppendorf







  -----Ursprüngliche Nachricht-----
  Von: Jenny Cabanillas [mailto:cabanillas@optonline.net]
  Gesendet: Donnerstag, 11. Mai 2006 21:43
  An: Cytometry Mailing List
  Betreff: FW: Magnetic cell sorting


  Dear Roger,

  I don’t believe we had the pleasure of meeting.  I am part of the
Invitrogen CSA team, and I’m responsible for the Dynal Beads in NJ/NY.

  It amazing that you would say 90% purity and recovery since from my
canvassing I have heard from researchers that the results vary incredibly
from

  Experiment to experiment (that’s if the columns don’t clog of course).  It
varies from applications but over 90% is a bit high. I would check my data
if I were you J



  Proof is in the pudding! Having fun selling Dynal beads,

  Jenny Cabanillas-McCauley









  -----Original Message-----
  From: Roger Burger [mailto:rburger@miltenyibiotec.com]
  Sent: Tuesday, May 09, 2006 5:44 PM
  To: Cytometry Mailing List
  Subject: RE: Magnetic cell sorting



  Hello Beverly

  I hope you meant to say,

  "Hah, you are lucky, for mouse the yield is more like 10% and that's what
Dynal tells you to expect with their system!"

   With the Miltenyi System and MACS®  CD4 and CD8 Microbeads you can
achieve >90% purity and >90% recovery.

  Regards,



  Roger A. Burger, Ph.D.
  Technical Marketing Scientist
  Miltenyi Biotec, Inc.
  12740 Earhart Ave.
  Auburn, CA 95602
  Phone: 530-887-5351
  Fax: 530-745-2851
  www.miltenyibiotec.com





  -----Original Message-----
  From: Beverly Barton [mailto:bartonbe@umdnj.edu]
  Sent: Tuesday, May 09, 2006 5:00 AM
  To: Cytometry Mailing List
  Subject: Re: Magnetic cell sorting



  Hah, you are lucky, for mouse the yield is more like 10% and that's what
Miltenyi tells you to expect with their system!



  Sad but true.....



  Beverly



  Beverly E. Barton, Ph.D.
  Assistant Professor
  Department of Surgery/Division of Urology
  UMDNJ-NJMS MSB G519
  185 S. Orange Avenue
  Newark, New Jersey 07103



  Telephone 973-972-0662
  E-mail bartonbe@umdnj.edu
  Telefacsimile 973-972-3892






  On May 6, 2006, at 3:50 AM, RAJA ALAMELU wrote:



  Dear Flow list members,

  My question is not directly related to flow cytometry, but now and then I
have seen questions in other areas also pop up.


  We are attempting to do CD4 and CD8 cells positive isolation from human
PBMC, followed by Detach-a bead step, using Dynal magnetic beads. We have
followed the manufacturers instructions as it, is as well as varied each of
the conditions for optimization.


  While the purity and viability after separation seem to be good, the final
cell yield never crosses ~30% of the total available CD4/CD8 in PBMC. Also
the initial input number, magnet bound cells and unbound cells do not tally.


  Is 25-30% the commonly expected yield using this technique? If anybody has
observed better yield, purity and viability, can you please share the
procedure?


  Thanking you in advance,
  Alamelu Raja



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