Dear Flowers, I recently have done three dye CTL assay on the flow cytometry for several times. The same problem seems to happen every time. It bothers me very much. The three dyes I used are the same: CFSE, PI and Cy5. I have every single staining for setting up the machines and compensation. But it turns out that it is very hard to adjust the parameters to make all the graphs looks right. If I adjust the voltage to make one dye into the scale in one graph, the dye will be out of scale in the other graph. Somebody told me only to use the three dyes graph as final adjustments. But I wonder if the three dyes are compatible. Can I adjust the amount of dye to make this better? The filters I used are PI 575/26, CFSE 530/30 and Cy5 660/20. I will appreciate any of your advices. Dept. of Infectious Diseases Veterinary School of Medicine Uinversity of Georgia, Athens,GA Phone: 706-2480026 Email:wenliang@uga.edu Homepage: http://www.arches.uga.edu/~wenliangReceived on Mon May 15 16:02:18 2006
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