PI has a tradition in eukaryotic biology as an indicator of cell death through membrane disruption. I am interested in whether any list watchers can tell me of cases in which strong PI staining is not an indicator of cell death. If many examples of PI staining of growing (or growth-capable) cells exist, then of what is PI staining indicative other than a (transient) membrane permeability difference compared to untreated controls? FYI, my definition of cell death is the inability of cells to divide when provided with appropriate conditions. I am less interested in other measurements of viability - "dividing or dead" is my motto... Thanks for your opinions..... Rob Palmer -- Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396Received on Mon May 1 10:38:00 2006
This archive was generated by hypermail 2.1.8 : Wed May 03 2006 - 04:12:02 EDT
