I have a Ph.D student want to detect her gelatin microsphere+BSA-FITC on Canto(See the following email and attachment. I don't know how to answer her question. Any information would be appreciated. Lisa > Hi Dear Lisa > If you remember I'd like doing flow cytometry for my microspheres. They are gelatin > microspheres.I want to know if I incubate with BSA-fitc ,How much BSA-FITC > will absorb to them.But the problem is that gelatin itself has fluorescence > in most wavelenghth specially fitc one.I attached the fluorescence photos > from gelatin microsphere and gelatin microsphere+BSA-FITC.Is it possible to > consider gelatin microspheres as a negative control.it's better for me not > to change the dye(fitc). > I'll be so appreciated if you help me > Have a nice day > Elham -- ******************************** BC Research Institute, UBC Room 212, 950 W 28th Ave. Vancouver, BC V5Z 4H4 Canada Tel: 604-875-2000 ext 5987 Fax: 604-875 3597 This attachment - 'BSA-FITC, 10x , 10ms.tif' - 4.21 MBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/8735c241afbcb8e458074d87838e94794dc06dba.tif This attachment - 'Gelatin-MS 10x , 100ms.tif' - 4.21 MBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/55cc2e3314ced216f3b7faf6494ab8598efe1251.tif
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