Alice, Qdots ranging from 800-545nm are excited by the violet laser (407nm) and these are measured using the optical setup as outlined in our web-site (http://www.niaid.nih.gov/vrc/labs_flow.htm). Mabs can be conjugated directly to the Qdots using the kits as discussed in their website ( http://probes.invitrogen.com/products/qdot/). Some of these are available with SA, which as you know can be bound to biotinylated Mabs. I would recommend reading the Qdot antibody conjugation kit user manual ( http://probes.invitrogen.com/media/pis/mp19010.pdf). Hope this helps SP On 4/14/06 4:10 PM, "Alice L. Givan" <Alice.L.Givan@Dartmouth.EDU> wrote: > Hello, > Can people tell me how you are staining cells with antibodies and the Quantum > Dot > fluorochromes? Are you conjugating the dyes to the antibodies yourselves > (using reagents > from where)? Or are you limiting yourselves to one Quantum Dot color with the > biotin/streptavidin combination? Or are you using different animal-derived > antibodies > with animal-specific secondary antibodies (from where)? Or what am I > missing? The goal > is to use lots of QDot colors with just 407nm excitation -- but I don't know > how close we > are to being able to do this. > > Thanks. > > Alice > > Alice L. Givan > Englert Cell Analysis Laboratory > of the Norris Cotton Cancer Center > Dartmouth Medical School > Lebanon, NH 03756 USA > tel 603-650-7661 > fax 603-650-6130 > givan@dartmouth.edu > www.dartmouth.edu/~celllab Stephen P. Perfetto, MS.,MT. (ASCP) Manager, Core Flow Cytometry Facility Vaccine Research Center, NIH Building 40 40 Convent Dr., Room 5507 Bethesda, MD 20892-3015 email: sperfetto@nih.gov Phone: (301) 594-8659 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. The National Institute of Allergy and Infectious Diseases (NIAID) shall not accept liability for any statement made that are the senderıs own and not expressly made on behalf of the NIAID by one of its representatives.Received on Tue Apr 18 14:18:00 2006
This archive was generated by hypermail 2.1.8 : Thu Apr 20 2006 - 04:12:02 EDT
