RE: Quantum Dots

You can conjugate antibodies directly with different colors of Quantum
dots using the conjugation kits from Quantum dots Co.  The conjugation
is not too difficult to follow the protocol that comes with the kits.
Using biotin/streptavidin or secondary antibodies methods are often more
difficult (if not possible) in multi-color staining than using the
direct conjugation methods.

Our experience of Qdots imaging indicated that higher concentration of
Qdots-conjugated antibodies are often needed in intracellular staining
than the use of the same antibody but conjugated with organic
fluorochromes.	Therefore, titration experiment with control samples are
needed.

Hope that helps.

Jian

*************************************
Jian Ling, Ph.D.
Principal Engineer
Bioengineering Section
Southwest Research Institute
6220 Culebra Rd.
San Antonio, TX 78238
Tel.  (210) 522 - 3953
Fax. (210) 684 - 6147
Email. jling@swri.org
Website: www.swri.org or
www.bioengineering.swri.org
*************************************



-----Original Message-----
From: Alice L. Givan [mailto:Alice.L.Givan@Dartmouth.EDU] 
Sent: Friday, April 14, 2006 3:10 PM
To: cyto-inbox
Subject: Quantum Dots


Hello,
Can people tell me how you are staining cells with antibodies and the
Quantum Dot
fluorochromes?	Are you conjugating the dyes to the antibodies
yourselves (using reagents
from where)?  Or are you limiting yourselves to one Quantum Dot color
with the biotin/streptavidin combination?  Or are you using different
animal-derived antibodies
with animal-specific secondary antibodies (from where)?  Or what am I
missing?	The goal
is to use lots of QDot colors with just 407nm excitation -- but I don't
know how close we are to being able to do this.

Thanks.

Alice

Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, NH 03756 USA
tel 603-650-7661
fax 603-650-6130
givan@dartmouth.edu
www.dartmouth.edu/~celllab