Three color flow cytometry!!! Help needed :)

From: Oliveira, Lilian <oliveira@animal.ufl.edu>
Date: Tue Apr 18 2006 - 09:28:38 EDT
Hello everyone!
I'm Lilian Oliveira and I'm trying to study Treg cells in livestock animals, so I have a
very basic question for you about which fluorochrome I should used in my experiments.
I have a anti CD25 that is IgG2a unconjugated, and I want to label my cells for FoxP3 and
CD4 just to start to search these cells in animal model.
My FoxP3 antibody is labeled with Zenon Alexa fluor 488, and my CD4 is PE labeled....I
really don't know which Zenon labeling I have to buy in order to proceed three color
studies....if somebody can help me...
I'm a Ph. D. student, and I'm starting to work with Flow Cytometry....I'm so glad to
enjoy in this list, I'm learning a lot with you....
Thanks in advance
Lilian Oliveira
Received on Tue Apr 18 12:18:00 2006

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