Re: Quantum Dots

Hi Alice,

We currently are using only one QDOT with our 407nm laser on the CyAn as we
have commercially available, and cheaper, fluorochromes in the other 8
channels.  We use the streptavidin conjugate and a biotin conjugated
primary.

Randy T. Fischer, NIH/NIAMS
B Cell Biology Group
9000 Rockville Pike
Bldg 10, Room 6D50
Bethesda, MD 20892
(301) 594-3537 (voice)
(301) 402-2209 (fax)


> From: "Alice L. Givan" <Alice.L.Givan@Dartmouth.EDU>
> Date: 14 Apr 2006 16:10:08 EDT
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Quantum Dots
> 
> Hello,
> Can people tell me how you are staining cells with antibodies and the Quantum
> Dot
> fluorochromes? Are you conjugating the dyes to the antibodies yourselves
> (using reagents
> from where)?	Or are you limiting yourselves to one Quantum Dot color with the
> biotin/streptavidin combination?  Or are you using different animal-derived
> antibodies
> with animal-specific secondary antibodies (from where)?  Or what am I
> missing? The goal
> is to use lots of QDot colors with just 407nm excitation -- but I don't know
> how close we
> are to being able to do this.
> 
> Thanks.
> 
> Alice
> 
> Alice L. Givan
> Englert Cell Analysis Laboratory
> of the Norris Cotton Cancer Center
> Dartmouth Medical School
> Lebanon, NH 03756 USA
> tel 603-650-7661
> fax 603-650-6130
> givan@dartmouth.edu
> www.dartmouth.edu/~celllab