Date: Wed Apr 12 2006 - 16:37:07 EDT
Hi Robin, I plan to talk about the SP assay performance on the FACSAria at the upcoming ISAC meeting (next month) in Quebec, using a new 375 nm non-fiber coupled laser system currently under development . We're still "working on it", as it were... more data by then. Best regards, Joe Joseph Trotter Director, Cytometry / Research and Development Advanced Technology Group BD Biosciences - Pharmingen R&D tel: (858) 812-8946 cell: (858) 449-7338 fax: (858) 812-8979 10975 Torreyana Road, San Diego, CA 92121-1106 USA E-mail: Joe_Trotter@bd.com Website: www.bd.com "Stingley, Robin L" <StingleyRobinL@uams.edu> 04/10/2006 12:21 PM To Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc Subject SP on FACSAria Hello all, I?ve posted questions about using a FACSAria to sort side populations before, but haven?t ended up trying it yet. Thanks to everyone who responded, especially Derek Davies, I think I could get our Aria set up properly and give it a try. My new question comes from a new customer who is thinking of trying the SP sorting. He is concerned that we can?t find published research using the FACSAria for this purpose, and since our configuration will not be optimal (we have the 407 laser, not a UV), perhaps his results will be in question. I?ve found Derek?s paper (JIM 310:171-181, 2006) and one other that used a 407 laser, although I?ve only seen the abstract and don?t know if they used an Aria or not (Methods Mol Biol. 263:161-180, 2004). The question is, are there other papers out there where a FACSAria was used to sort side populations? I don?t think this customer is willing to be my guinea pig, and I really can?t blame him. Thanks in advance, Robin Robin Stingley, Ph.D. Flow Cytometry Core Facility Department of Microbiology and Immunology University of Arkansas for Medical Sciences Slot 511, Rm B504 4301 West Markham Street Little Rock, Arkansas 72205 Phone: 501-686-6927 E-mail: StingleyRobinL@uams.edu http://www.uams.edu/flowcytometry/ ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ ----------------------------------------- ************************************************** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A: This message may constitute an advertisement of BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to the optoutbygroup@bd.com. ************************************************** This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank You ************************************************** Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A.
