Dear Flowers, We are currently developing a B cell phenotyping assay for the diagnosis of CVID and other humoral Immunodeficiencies. I am suspecting that the cells that were cryopreserved are expressing a higher percentage of CD19+ b cells than the fresh ones. Have anyone seen such an effect?. I also request if anyone could give me an idea of the normal reference range of Cd19+ B cells and CD27+CD19+ B cells in PBMCs. We are currently measuring about 12-15% of CD19+ Cells (gated on lymphs using CD45) and among the B cells, we have about 10-20% of CD27 expression. Is this normal or are we doing something wrong? I thank you all in advance for your replies. Regards, Vijay Cellular and Molecular Immunology, Mayo Clinic, Rochester, MN
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