Hey Barry You should probably lower your pressure and increase your nozzle size. I would imagine quite a few cells perish when blasted out of the nozzle. Under 20psi and a 100u nozzle would be kinder to your cells. Best Simon Monard Trudeau Institute 154 Algonqin Ave Saranac Lake New York 12983 Ph 518 891 3080 >>> "Barry Moran" <barry.moran@tcd.ie> 04/05/06 11:40 am >>> Hi there- We're sorting cultured murine dendritic cells on a MoFlo (70micron nozzle, 60psi) into culture media. We're finding that the cells appear some- what activated (CD40+, CD80+, CD86+) after the sort compared to unsorted controls. Have many others found this, and if so are there any suggestions or fixes- is it likely to be endotoxins etc in the sheath fluid/lines? Many thanks Barry. Barry Moran Flow Cytometry Facility School of Biochemistry and Immunology 0.19, Biotechnology Building Trinity College Dublin +353 1 608 3575 barry.moran@tcd.ie <http://www.tcd.ie/biochemistry/flow> www.tcd.ie/biochemistry/flowReceived on Fri Apr 7 14:38:00 2006
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