[ Dendritic cell activation during sort ]

From: Barry Moran <barry.moran@tcd.ie>
Date: Wed Apr 05 2006 - 11:40:24 EDT
Hi there-
We're sorting cultured murine dendritic cells on a MoFlo (70micron nozzle,
60psi) into culture media. We're finding that the cells appear some-what
activated (CD40+, CD80+, CD86+) after the sort compared to unsorted
controls. Have many others found this, and if so are there any suggestions
or fixes- is it likely to be endotoxins etc in the sheath fluid/lines?
Many thanks
Barry.
Barry Moran
Flow Cytometry Facility
School of Biochemistry and Immunology
0.19, Biotechnology Building
Trinity College Dublin
+353 1 608 3575
barry.moran@tcd.ie
 <http://www.tcd.ie/biochemistry/flow> www.tcd.ie/biochemistry/flow
Received on Thu Apr 6 14:35:10 2006

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