My two cents: We are facing a similar type of problem with FACSCanto, which is sort of a simplified version of Aria for clinical applications. When height FSC is used to display the data, we see kind of a curtain, which makes the last decade of events invisible (cuts them off). When area FSC is used instead of height, it displays events distributed normally. According to BD recommendations, we tried to do the following exercise: change the slope between height and area to make these two identical. Apparently it did not help. From that time we have always used only FSC area. According to BD, this is not changing our final results. So I am not sure that it is appropriate to see different distributions of events and have no idea how to fix it. Irina Irina Grigorieva, PhD Director, Flow Cytometry Laboratory Northside Hospital, Atlanta, GA (404)- 851-6541 e-mail: irina.grigorieva@northside.com > -----Original Message----- > From: Joey Ilas [SMTP:joeyilas@gmx.net] > Sent: Monday, March 27, 2006 4:24 AM > To: Cytometry Mailing List > Subject: Area and Height Facs Aria > > Hi > > To Facs Aria user : > > I am confused with the data we aquired using area compared to height > measurement . I found very noticeable noise mostly with the negative > population . I run samples like mouse spleen cells and human PBMC , the > signal with the negative population shows diagonal spreading with area > while height is a quadratic population . > I verify this results using Calibrite beads but the same situation also > compared using mouse or human cells. > The aria was newly calibrated ( area scaling , laser delay , laser > calibration ) I asked the BD technicians about explanation but he could > not explain why . > > thanks > > Joey Ilas > Baxter AG > Immunology Dept. > Vienna Austria > > -- > Bis zu 70% Ihrer Onlinekosten sparen: GMX SmartSurfer! > Kostenlos downloaden: http://www.gmx.net/de/go/smartsurfer >Received on Tue Mar 28 18:05:48 2006
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