Hi J., It would be good to include some more detailed information. For instance, what "diagonal"? Height versus area? If so, on which parameter? FL1 vs. FL2? If the latter, what do your compensation controls look like? Did you pre-gate fluorescence measurements on forward/side scatter or not?... Regards, Guy ------------------------------------ Ablynx NV Guy Hermans, PhD Senior Scientist guy.hermans@ablynx.com Technologiepark 4 B-9052 Zwijnaarde Belgium tel: +32 (0)9 261 06 57 fax: +32 (0)9 261 06 27 mobile: +32 (0)486 788 551 ------------------------------------ -----Original Message----- From: Joey Ilas [mailto:joeyilas@gmx.net] Sent: Monday, March 27, 2006 11:24 AM To: cyto-inbox Subject: Area and Height Facs Aria Hi To Facs Aria user : I am confused with the data we aquired using area compared to height measurement . I found very noticeable noise mostly with the negative population . I run samples like mouse spleen cells and human PBMC , the signal with the negative population shows diagonal spreading with area while height is a quadratic population . I verify this results using Calibrite beads but the same situation also compared using mouse or human cells. The aria was newly calibrated ( area scaling , laser delay , laser calibration ) I asked the BD technicians about explanation but he could not explain why . thanks Joey Ilas Baxter AG Immunology Dept. Vienna Austria -- Bis zu 70% Ihrer Onlinekosten sparen: GMX SmartSurfer! Kostenlos downloaden: http://www.gmx.net/de/go/smartsurferReceived on Tue Mar 28 16:18:00 2006
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