RE: [mirobial] Harvesting bacteria from infected tissue for subsequent flow analysis

Following a workshop I gave at the EMBO course in Berlin in 2004
discussing bacterial heterogeneity in gene expression I spent some time
with Dirk Bumann at the Deutsche Rheumaforschungszentrum in Berlin. He
has published the work on Salmonella isolation doing exactly this:    

Nature 440, 303-307 (16 March 2006)

Robust Salmonella metabolism limits possibilities for new antimicrobials

Daniel Becker1
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a1
> ,5
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a5
> , Matthias Selbach2
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a2
> ,5
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a5
> , Claudia Rollenhagen1
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a1
> ,5
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a5
> , Matthias Ballmaier3
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a3
> , Thomas F. Meyer1
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a1
> , Matthias Mann2
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a2
>  and Dirk Bumann1
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a1
> ,4
<http://www.nature.com/nature/journal/v440/n7082/abs/nature04616.html#a4
> 

 
Regards
 
Gerhard
 
 
P.S.
 
Howard could we please agree to put [microbial] at the front of the
message so it is more useful in the "sort by name" functions
 
 
-----Original Message-----
From: Nicholas Lejarcegui [mailto:nicholas.lejarcegui@sbri.org] 
Sent: 22 March 2006 21:07
To: cyto-inbox
Subject: Harvesting bacteria from infected tissue for subsequent flow
analysis



	Hello, 
	I was asked the following question, and I am empty handed, but I
thought I would pass it on to the many out there who might have
knowledge in this area.  

	Here is the question 
	I was wondering if anyone had any experience with harvesting
bacteria from infected tissues for subsequent flow analysis? I'm sure
there's a way to do it, but before I jumped into a literature search I
thought I'd go to the folks who might know.

	Thanks, 
	Nick Lejarcegui  
	Nicholas.Lejarcegui@sbri.org 
	Seattle Biomedical Research Institute 
	Flow Cytometry Core Facility