digital data transformation revert

While we are on the subject.  I have listened to this discussion and 
have played around a bit with the transformation in flowjo using data 
acquired on our FACScanto Diva software.
How does one revert back to the original view without having to bring 
the file back in?


On Thursday, March 16, 2006, at 02:06  AM, A.J. Rossini wrote:

> On 3/13/06, Mario Roederer <roederer@drmr.com> wrote:
>> I am a firm believer in the use of these transformations--in the 
>> multicolor> world,
> they have revolutionized our ability to look at the data.  (Note that> 
> the transforms in
> no way affect statistics or gating--they ONLY affect the> visual 
> representation of the
> data).	The point of these transforms is to> make the data more easily 
> conveyed to
> readers--something that a simple log> scale (which is itself a 
> transformation!) no longer
> does adequately.
> I'm going to nitpick a bit -- while the general overall 
> conclusionsMario draws are
> correct, there are a few subtle points.   If you don'tget what I'm 
> saying, believe Mario,
> it's simpler that way.	Read onfor the fine details:
> Transformations actually do affect the statistical 
> analysis/conclusions.
> data != transformed data.
> Conclusions drawn on transformed data do not have to be the same as 
> onraw data (a
> difference on a log scale using a particular statisticisn't 
> necessarily different using
> the raw data with the samestatistic).
> Why transform?
> To pull the data back to a state of nature where we feel 
> comfortablewith decision making.
> To pull the data back to a state of nature where certain 
> probabilisticassumptions are
> met, allowing for "believable" statistical estimationand testing (and 
> opposed to
> unbelieveable statistical muck)
> To focus on particular ranges or features of the data.
> All are valid.
> The detail is just "comparison on the transformed scale isn't the 
> sameas comparison on
> the original scale".	The point is that it's ratherimportant to do, 
> both to match up
> with historical scientificapproaches as well as to match up with 
> common practices
> forcomparability within the cytometric field.
>>> The Data Presentation Standards Committee, formed by ISAC, will take 
>>> the>
> responsibility to contact the editors of the Journal with which there 
> are> problems.  Our
> goal is to educate not only the reviewers, but importantly> the 
> Editors--so that they can
> be made aware that any complaints that> reviewers might have regarding 
> this visualization
> are baseless.
> This is important!  Visualizations, done well, are critical 
> formultidimensional data.
> best,-tony
> (Novartis Pharma AG / University of Washington)
> blindglobe@gmail.comMuttenz, Switzerland."Commit early,commit often, 
> and commit in a
> repository from which we can easilyroll-back your mistakes" (AJR, 
> 4Jan05).
>
Julia Whynot
Rockefeller University
Investigative Dermatology
212-327-7581