We have been sorting trangenic gfp mouse embryonic spinal cord and adult brain cells. The spinal cord is fine with 70 micron orifice at 12 psi differential on the Vantage SE. For the adult brain cells, we have gone to a pre-enrichment using percoll prior to the sorts. Rochelle Rochelle (Shelley) Diamond Member of the Professional Staff California Institute of Technology Division of Biology Flow Cytometry Cell Sorting Facility 1200 E. California Blvd. Pasadena, CA 91125 626-395-4947 phone diamond@its.caltech.edu On Tue, 14 Mar 2006, Larry Arnold wrote: > Tim > > We sort mouse neuronal cells regularly on our MoFlo. We have found that > up through embryonic day 16 the cell preps are good and we can sort them > using a 70um tip. Day 17 and later require a 100um tip (to get good > tight side sort streams) and the cells preps become increasing poorer > with increasing age (probably due to disaggregation difficulties) and > with lots of debris. > > Larry > > At 04:01 PM 3/14/2006, you wrote: > > Hello all: > > This is cross-posted from the Purdue cytometry list: > > Most of the sorting I do falls into two areas hematopoietic > cells and transfected cell lines. I have several > investigators who are interested in sorting various neuronal > precursor cells. > > I’ve been told (anecdotally) that sorting neuronal cells is > difficult and requires considerations different from sorting > neuronal cells. I was wondering if anyone out there has any > experience and can offer some guidance in sorting these > cells, especially on a FACSAria. > > Thanks in advance! > > Regards, > > Tim > > > Timothy Bushnell, Ph.D. > > Director, CPBR Flow Lab > > University of Rochester > > tel: 585-273-1361 > > fax: 585-276-0233 > > http://www.urmc.rochester.edu/Aab/geneped/flow/ > > http://www.urmc.rochester.edu/wnyfug/ > > > > Use of the return email address on this message for > commercial purposes is prohibited. The transmission of > unsolicited commercial material is prohibited under federal > laws (47 USC 227). Civil penalties and claims of $500.00 per > occurrence (47 USC 227[c]) may be assessed for violations. > > Larry W. Arnold, Ph.D. > Research Professor and Director, Flow Cytometry Facility > Department of Microbiology and Immunology > CB# 7290 > University of North Carolina > Chapel Hill, NC 27599 > Phone: 919-966-1530 > FAX: 919-962-8103 >Received on Thu Mar 16 16:18:00 2006
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