Dear Flowers, I have one more question with regards to the CFC(Cytokine Flow Cytometry) using the Canto. What would be the best viability dye that can be used in a Canto. I would like to use the Blue Laser Detector A (far red, detector for PE-Cy7) as I have other colors filling up other channels. I am looking for a viability dye that can be collected in this detector and also pose no problems with perm/fix protocols. I request all of you to suggest me a useful viability dye for the FACS canto. I tried using the 7-AAD but could not quite differentiate the dead cells as I guess the perm procedure is causing a problem. Waiting for your suggestions. Thanks, Vijay
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