Viability Dye for the Canto

From: Nandakumar, Vijay <Nandakumar.Vijay@MAYO.EDU>
Date: Mon Feb 27 2006 - 16:14:51 EST
Dear Flowers,
I have one more question with regards to the CFC(Cytokine Flow Cytometry) using the
Canto. What would be the best viability dye that can be used in a Canto. I would like to
use the Blue Laser Detector A (far red, detector for PE-Cy7) as I have other colors
filling up other channels. I am looking for a viability dye that can be collected in this
detector and also pose no problems with perm/fix protocols. 
I request all of you to suggest me a useful viability dye for the FACS canto. I tried
using the 7-AAD but could not quite differentiate the dead cells as I guess the perm
procedure is causing a problem.
Waiting for your suggestions.
Thanks,
Vijay




Received on Tue Feb 28 11:38:00 2006

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