Dear Flowlosophers: I had always wondered about the issue of distilled water verses Isoflow as a sheath fluid. While I was reading all 31 responses a wild a crazy thought occurred to me----Do the experiment and see! Using a FASCalibur and a FACScan, the latter of which has been recently rescued from storage and needs a new flow cell, I ran BD Calibrite beads, unlabelled and PE-labeled, diluted in IsoFlow (Coulter) and compared the readings using IsoFlow as Sheath to Water as Sheath. The water was house DI water filtered with a .22micron filter before use. Detector settings were left unchanged as the filter and system was flushed with its new sheath fluid. The results which are attached as a PowerPoint showed that there was no appreciable difference in sensitivity or precision between the two sheath fluids. However, there was a noticeable increase in the noise detected in a scatter plot using water as a sheath. It seems to me that water is perfectly adequate as a sheath fluid for ordinary work where you can gate out the noise using a live cell gate, as is normally done. Hope this is worth all the Out of Office responses I'm going to get! Sincerely, Dan Rosson PhD FlowJock Thomas Jefferson University You can observe a lot by watching. --Y. Berra This attachment - 'DIWater&sheath.ppt' - 87.04 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/42884bf8d3aa8178f6a1fd31b4e3b235f379fb5c.pptReceived on Tue Feb 28 10:38:00 2006
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