CD3 activation of T cells for cytokine staining

Flowers,

I have used just about every stimulus (PMA/iono, Ag, super-Ag, LPS, etc.) to activate
cells for intracellular cytokine staining EXCEPT anti-CD3. I would like to activate human
T cells with anti-CD3 for intracellular cytokine staining and/or Miltenyi cytokine
secretion assay. 

Question: Does anyone have experience using anti-CD3 activation of human T cells in these
assays? Soluble vs. plate bound vs. beads? Particular clones that are better? My major
interest is Th2 cytokines, but I'll take any advice.

On a similar note, does anyone know of a source for the 64.1 anti-huCD3 hybridoma? I'd
love to get my hands on the cells....

Thanks!

Calman
> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> National Institute of Allergy and Infectious Diseases/ NIH
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