Hi everyone. One of my colleagues has asked me if it would be possible to use an anti-GFP antibody labelled with Alexa 488 to amplify the signal from intracellular GFP production in a special strain of mice we have here. Is this possible? Alexa 488 emits at 519nm and GFP emits at 509nm (wavelengths pulled from literature) therefore they would both be picked up in our FACsCalibur 530/30 filter. Would there be a problem with quenching? Or is there a better way to amplify weak signals. Thanks for your time Kylie Price Staff Scientist Flow Cytometry The Malaghan Institute of Medical Research The Central Services Building Victoria University Campus Entrance 7 Kelburn Parade Wellington New Zealand Ph: 64-4-4996914, ext 850 Fax: 64-4-4996915 E-mail: kprice@malaghan.org.nz Web: www.malaghan.org.nz ************************************************************************ The information contained in this message and any attachments may be confidential and privileged and is intended for the addressee(s) only. If you are not the intended recipient of this message please respect the confidentiality of, and do not disclose, copy or disseminate, the contents. If you have received this message in error then please notify the sender immediately. Opinions, information or any other comments expressed in this message are not necessarily held or endorsed by the Malaghan Institute of Medical Research. ************************************************************************Received on Wed Feb 15 14:18:00 2006
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