Hi Bill, I've seen a DakoCytomation tech support/sales person use an Excel sheet for this purpose. Although probably MoFlo specific, I expect this would give you a fair estimate for other brand instrument/nozzles etc. as well. I don't have a copy of this document, but would be interested in getting one as well (or a similar one if BD has one - HINT!). regards, Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: William Throndset [mailto:william.throndset@danisco.com] Sent: Wednesday, February 08, 2006 11:36 PM To: cyto-inbox Subject: droplet volumes Hello, Can anyone tell me the volume of droplets coming out of a jet-in-air nozzle? I am usually using a 70 micron nozzle at 60psi and about 100,000 droplets per second. I'd also like to know how to calculate this myself. Also, since the droplets are made up of sheath and sample, what portion of each droplet is sheath, and what portion is sample? Thanks, Bill Throndset Genencor International Palo Alto, CA ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
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